Evaluation of pre-analytical factors affecting plasma DNA analysis

Pre-Analytical Parameters Affecting Vascular Endothelial Growth Factor Measurement in Plasma: Identifying Confounders

BACKGROUND Vascular endothelial growth factor-A (VEGF-A) is intensively investigated in various medical fields. However, comparing VEGF-A measurements is difficult because sample acquisition and pre-analytic procedures differ between studies. We therefore investigated which variables act as confounders of VEGF-A measurements. METHODS Following a standardized protocol, blood was taken at three...

Critical care analytes: pre-analytical factors affecting result quality for combined blood gas and electrolyte systems

Experimental factors affecting the robustness of DNA methylation analysis

Diverging methylation frequencies are often reported for the same locus in the same disease, underscoring the need for limiting technical variability in DNA methylation analyses. We have investigated seven likely sources of variability at different steps of bisulfite PCR-based DNA methylation analyses using a fully automated quantitative methylation-specific PCR setup of six gene promoters acro...

Analysis of Factors Affecting the Ecotourism Development

This research was carried out to investigate the role of natural resources cooperatives in ecotourism development. This was an analysis-descriptive study conducted through survey method. Statically population of study was 123 members of the directors of these cooperatives. A structured questionnaire was designed and distributed among the cooperative directors. Census technique was applied in th...

Influence of Pre-Analytical Factors on Thymus- and Activation-Regulated Chemokine Quantitation in Plasma

Thymus- and activation-regulated chemokine (TARC) in serum/plasma associates with the disease activity of atopic dermatitis (AD), and is a promising tool for assessing the response to the treatment of the disease. TARC also exists within platelets, with elevated levels detectable in AD patients. We examined the effects of pre-analytical factors on the quantitation of TARC in human EDTA plasma. ...